Course Details
Course Outline
5 days course
Introduction to Chromatography
- The History of Chromatography – GC & LC
- Overview of GC & LC Chromatography
- The Modern Chromatograph
- Liquid Chromatography – The Development Process
- Factors Controlling Retention
- Molecular Forces and Chromatographic Selectivity
- Effects of Stationary Phase Loading on the Performance of a Chromatographic System
- Chromatography Nomenclature
Chromatography Basics
- Basic Chromatography
- Sample Introduction
- The role of sample introduction and injection ports in GC operations
- Injection ports maintenance and its impact on GC performance
- Columns
- The role of columns in GC operations.
- Column selection & maintenance
- How columns can impact GC performance
GC Operation
- Peak Dispersion in a Chromatographic Column
- Detector Selection
- The role of Detectors in GC operations
- Detector maintenance
- How detectors can impact GC performance
- Setup and GC. Operation, Basic steps
- Preparation for operation.
- Sampling Techniques
Data Acquisition and Calibration
- Calibration
- Data Acquisition and Processing System
- Calibration linked to GC performance
Applications and troubleshooting
- Chromatography Applications
- Method Development
- Gas Chromatography
- Liquid Chromatography
- ISO17025 Accreditation Basics
- Laboratory Management & Troubleshooting
